So my last post was about how charged up I am about an academic career.
With that in mind, let me describe my week:
Saturday: Transfected cells, let's say A cells, to use for experiments. Fed them, put them away, felt very good about the adventurousness of the experiment being undertaken.
Sunday: Realized while 40 miles away that I had completely forgotten to get another set of cells, call them B cells, ready for the experiment. B cells are needed for the final step of the experiment. Crap. Bummer. Well will just have to get them ready on Monday.
Monday: Got B cells, and third and final type of cells, called C, ready. A cells, of course are nice and confluent and ready to use, only I am not going to use them. F*cking A. Efficiently set up more of A cells to start the whole things again, but nested so I don't waste any time.
Tuesday: Set it all up, A cells, B cells and C cells. They are happy in the incubator, ready for me to read them tomorrow. Whew, that didn't work out too badly did it?
Transfected second batch of A cells.
Wednesday: Started off readings, all negative. Completely, and it doesn't even look like A cells make the protein I transfected into them (which is the whole entire point of transfection!). Double sh*t. No f*ck it, triple sh*t. Then the blinding realization dawns that I threw away my leftover B cells after using them yesterday, and I don't have any more going. There are no words, only anti-endorphins.
Thursday: Started round 2, with some alternative B cells. Also, smartly decided to check whether transfecting served any purpose this time, since I screwed up the previous time and let A cells go much longer than they should have. Turns out that transfecting A cells doesn't work when I do it (though it is routine procedure in my lab). Nothing, nothing at all. There's no point in doing the next step, with alternative or otherwise B cells. Now what? Blog, I suppose. Maybe some beer.
Friday: Strategize? Re-evaluate? Retire?
I am a shining example, I tell you.